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Journal / Biochemistry / 荧光RNA适配体 外和细胞…
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Biochemistry

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JoVE Journal Biochemistry
荧光RNA适配体 外和细胞 开启动力学的测定
 
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荧光RNA适配体 外和细胞 开启动力学的测定

Article DOI: 10.3791/64367-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science, University of Utah
* These authors contributed equally

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该协议提供了两种确定荧光RNA适配体菠菜2和西兰花动力学的方法。第一种方法描述了如何使用酶标仪 在体外 测量荧光适配体动力学，而第二种方法详细介绍了通过流式细胞术测量细胞中荧光适配体动力学。

Tags

生物化学，第 186 期，荧光，流式细胞术，酶标仪，菠菜2适配体，西兰花适配体， 大肠杆菌
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