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从人窦卵泡中提取、标记和纯化谱系特异性细胞
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JoVE 신문 생물학
Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles
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从人窦卵泡中提取、标记和纯化谱系特异性细胞

DOI:
10.3791/64402-v

•

06:36 min

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November 30, 2022

•

, , , , ,
1Center for Reproductive Medicine and Infertility,Weill Cornell Medical College, 2Tri-Institutional Stem Cell Derivation Laboratory,Weill Cornell Medical College

Chapters

  • 00:05Introduction
  • 00:45Processing the Ovarian Tissue and Isolation of Antral Follicles
  • 01:50Isolation of Follicle Resident Cells
  • 03:01Fluorescence-Activated Cell Sorting
  • 03:36Processing of the Ovarian Cortical Tissue and Ovarian Tissue Slow Freezing
  • 04:24Results: Identification and Purification of Ovarian Cells from Antral Follicles
  • 05:45Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出了从窦卵泡中鉴定和纯化卵巢细胞的方案。我们详细说明了处理整个卵巢以冷冻保存皮质条的方法，同时还收获完整的窦卵泡，这些卵泡经过酶处理以释放多种卵泡常驻细胞类型，包括颗粒细胞、膜细胞、内皮细胞、造血细胞和基质细胞。

Tags

Keywords: Ovarian FolliclesAntral FolliclesGranulosa CellsStroma CellsTheca CellsCell IsolationCell LineageCell PurificationEnzymatic DigestionFluorescent LabelingFlow Cytometry

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