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A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
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A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

DOI:
10.3791/64418-v

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08:24 min

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November 25, 2022

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, ,
1Department of Biological Sciences,Ajou University

Chapters

  • 00:05Introduction
  • 00:48Yeast Transformation and Spotting Assay
  • 02:57Measurement of Yeast Growth Using a Microplate Reader
  • 04:18Fluorescence Microscopy
  • 06:31Results: Cytotoxicity of α-Synuclein on Agar and Liquid Media and Analysis of Yeast Cells by Fluorescence Microscopy
  • 07:28Conclusion

Summary

자동 번역

자동 번역

An in vivo physiological model of α-synuclein is required to study and understand the pathogenesis of Parkinson's disease. We describe a method to monitor the cytotoxicity and aggregate formation of α-synuclein using a humanized yeast model.

Tags

Keywords: α-synucleinYeast ModelParkinson’s DiseaseProtein AggregationCellular PhenotypesGenetic FactorsGrowth AssayToxicity

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