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Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 01:39Preparation of Complete Barbour-Stoenner-Kelly (BSK)-II Medium
- 02:48Culturing Borrelia with or Without Antibiotics and Long-Term Storage
- 05:48Isolation of Spirochetes from Environmental or Clinical Sources
- 07:30Isolation of Monoclonal Populations of B. burgdorferi Sensu Lato Spirochetes and B. miyamotoi from Liquid Cultures by Cultivation on Solid Media
- 08:39Results: Culturing of Diverse Borrelia Spirochetes
- 09:23Conclusion
In vitro culture is a direct detection method for the presence of living bacteria. This protocol describes methods for the culture of diverse Borrelia spirochetes, including those of the Borrelia burgdorferi sensu lato complex, relapsing fever Borrelia species, and Borrelia miyamotoi. These species are fastidious and slow growing but can be cultured.
