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从小鼠子宫建立3D子宫内膜类器官
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Establishing 3D Endometrial Organoids from the Mouse Uterus
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从小鼠子宫建立3D子宫内膜类器官

DOI:
10.3791/64448-v

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06:24 min

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January 06, 2023

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, , , , ,
1Department of Pathology & Immunology,Baylor College of Medicine, 2Center for Drug Discovery,Baylor College of Medicine, 3Departments of Molecular Virology and Microbiology and Medicine,Baylor College of Medicine

Chapters

  • 00:05Introduction
  • 00:40Isolation of Uterine Epithelium from Mice Using Enzymatic and Mechanical Methods
  • 03:18Encapsulation of Uterine Epithelium into Gel Matrix to Establish Organoids
  • 04:19Results: Gene Expression and Histological Analyses of Mouse Endometrial Epithelial Organoids
  • 05:32Conclusion

Summary

자동 번역

자동 번역

该协议描述了建立小鼠子宫内膜上皮类器官以进行基因表达和组织学分析的方法。

Tags

3D Endometrial OrganoidsMouse UterusUterine EpitheliumEnzymatic DissociationMechanical DissociationCell CultureOrganoid EstablishmentCell PelletCollagenaseDNA Solution

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