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用于使用受激拉曼散射显微镜进行延时活细胞成像的柔性腔室
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JoVE 신문 생체공학
A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
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用于使用受激拉曼散射显微镜进行延时活细胞成像的柔性腔室

DOI:
10.3791/64449-v

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07:40 min

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August 31, 2022

•

,
1Department of Biomedical Engineering, Binghamton University,State University of New York

Chapters

  • 00:05Introduction
  • 00:48Microscope Environmental Enclosure
  • 01:56Assembly of the Flexible Chamber
  • 03:09Preparation for Time-Lapse Live-Cell SRS Imaging
  • 04:08Time-Lapse Live-Cell SRS Imaging
  • 05:25Results: Time-Lapse SRS Imaging of Live SKOV3 Cells
  • 07:01Conclusion

Summary

자동 번역

자동 번역

我们报告了一种台顶柔性环境室，用于使用具有透射信号检测的正置受激拉曼散射显微镜对活细胞进行延时成像。脂质液滴在用油酸处理的SKOV3细胞中成像长达24小时，时间间隔为3分钟。

Tags

Flexible Environmental ChamberTime-lapse Live-cell ImagingStimulated Raman Scattering (SRS) MicroscopyTransmitted Signal DetectionHigh Numerical Aperture ObjectiveHigh-end CondenserSilicone Rubber SheetMica Ceramic SheetsPolycarbonate SheetsFlexible Duct HoseHeater ModuleMachined Aluminum PiecesNatural Rubber Film SleeveCO2 CylinderGas Mixer ModuleInline FiltersPre-sterilized Water BottleHotplate

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