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O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy
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O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy

O-Ring Aortic Banding Versus Traditional Transverse Aortic Constriction for Modeling Pressure Overload-Induced Cardiac Hypertrophy

DOI:
10.3791/64455-v

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09:24 min

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October 06, 2022

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, , , , , , , ,
1Department of Internal Medicine III, German Centre for Cardiovascular Research,University Hospital Kiel, Partner Site Hamburg/Kiel/Lübeck, 2Internal Medicine III, German Centre for Cardiovascular Research,University Hospital Heidelberg, Partner Site Heidelberg/Mannheim

Chapters

  • 00:04Introduction
  • 00:48Preparation of the O-Ring
  • 01:37Premedication of the Mice and Preparation of the Operating Field
  • 02:10Intubation of the Mice
  • 03:33Surgery and Preparation for Ring Positioning
  • 04:41Ligation of the Transverse Aorta with the O-Ring
  • 05:41Suture and Post-Operative Recovery
  • 06:33Confirmation of Successful Constriction and Right Position of the Ring
  • 08:08Results: Modeling Pressure Overload-Induced Cardiac Hypertrophy Using the O-Ring Aortic Banding in a Murine Model
  • 08:57Conclusion

Summary

자동 번역

자동 번역

The present protocol describes a new technique of aortic banding in mice to induce pressure overload cardiac hypertrophy. For banding, a rubber ring with a fixed inner diameter is used. This new technique promises less variance and more reproducible data for future experiments.

Tags

O-ring Aortic BandingPressure OverloadCardiac HypertrophyAortic ConstrictionMouse ModelMicrosurgeryAnesthesiaIntubationHeating PadVentilator

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