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体外研究 研究结膜杯状细胞中基于性别的差异的方法
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In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells
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体外研究 研究结膜杯状细胞中基于性别的差异的方法

DOI:
10.3791/64456-v

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06:28 min

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July 28, 2023

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, ,
1Department of Ophthalmology Schepens Eye Research Institute, Massachusetts Eye and Ear,Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:40Primary Human Conjunctival Goblet Cell Culture and Passaging
  • 02:52Fura‐2/Acetoxymethyl (AM) Assay for [Ca2+]i Measurement
  • 04:52Results: Measuring [Ca2+]i Concentration in Human Conjunctival Goblet Cells
  • 05:30Conclusion

Summary

자동 번역

자동 번역

在基于性别的差异研究中，无酚红/无胎牛血清培养基是比晚期 RPMI 更好的选择，可在不改变结膜杯状细胞正常功能的情况下消除外源激素。

Tags

In VitroConjunctival Goblet CellsSex-based DifferencesPrimary CultureIntracellular Calcium MeasurementFura-2 AMOcular Surface DiseasesDrug DevelopmentCell CultureTrypsinization

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