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Chapters
- 00:04Introduction
- 00:38Fabrication of the Two-Piece Channel and Modular Platform Base
- 04:28Injecting the COL1 Solution into the Microchannel and Removing the Cover for Cell Culture Applications
- 06:16Results: Confocal Reflectance Images of COL1 Fibers
- 06:45Conclusion
This protocol demonstrates the use of a microfluidic channel with changing geometry along the fluid flow direction to generate extensional strain (stretching) to align fibers in a 3D collagen hydrogel (<250 µm in thickness). The resulting alignment extends across several millimeters and is influenced by the extensional strain rate.
