Quantification of Circular RNAs Using Digital Droplet PCR

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Quantification of Circular RNAs Using Digital Droplet PCR

DOI:

10.3791/64464-v

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08:39 min

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September 16, 2022

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Arundhati Das*^1,2, Debojyoti Das*^1,2, Amaresh C. Panda

¹Institute of Life Sciences, ²School of Biotechnology,KIIT University

Chapters

00:04Introduction
01:02Divergent Primer Design for circRNA and RNA Isolation
03:16cDNA Synthesis
04:09Digital Droplet PCR (dd-PCR) Workflow
07:04Results: Data Analysis Using QuantaSoft Software
08:05Conclusion

Summary

자동 번역

This protocol describes the detailed method of digital droplet PCR (dd-PCR) for precise quantification of circular RNA (circRNA) levels in cells using divergent primers.

Quantification of Circular RNAs Using Digital Droplet PCR

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Chapters

Summary

자동 번역

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