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マウスにおける肝部分切除後の再生肝細胞の単離
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의학
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JoVE 신문 의학
Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice
DOI:

10:04 min

December 02, 2022

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Chapters

  • 00:04Introduction
  • 00:43Preparation of Perfusion Equipment and Animal Surgery
  • 02:51Cannulation and Perfusion
  • 05:03Digestion
  • 06:21Preparation of the Liver
  • 06:51Hepatocyte Extraction and Isolation
  • 08:11Results: A Reliable and Straightforward Method for Isolating High Yield Normal and Steatotic Murine Hepatocytes
  • 09:37Conclusion

Summary

자동 번역

脂質を含んだ肝細胞は肝臓の再生に固有のものですが、通常は密度勾配遠心分離で失われます。ここでは、脂肪肝細胞を保持する最適化された細胞分離プロトコルを提示し、マウスの部分肝切除後に再生肝細胞の代表的な集団を生み出します。

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