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Culture cellulaire IDG-SW3 dans une matrice extracellulaire tridimensionnelle
JoVE 신문
생체공학
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JoVE 신문 생체공학
IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
DOI:

10:48 min

November 13, 2023

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Chapters

  • 00:05Introduction
  • 00:33Preparation of the Cell-Matrix Mixture
  • 02:07Plating the Cell-Gel Mixture
  • 02:58Identifying the Cell Viability and Cellular Dendrites Using a Confocal Microscope
  • 06:00Identifying Appearance Using a Stereomicroscope
  • 06:55Identifying Mineral Deposition by XRF Assay
  • 07:45Identifying Functional Gene Expression
  • 08:25Results: Dendrite Network, Mineral Deposition, and Gene Expression of Osteogenic Cultured IDG-SW3 Cells
  • 10:04Conclusion

Summary

자동 번역

Nous présentons ici un protocole de culture de cellules IDG-SW3 dans une matrice extracellulaire tridimensionnelle (3D).

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