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Biochemistry

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JoVE Journal Biochemistry
Live-Cell-Bildgebung intakter Ex-vivo-Globen mit einem neuartigen 3D-gedruckten Halter
 
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Live-Cell-Bildgebung intakter Ex-vivo-Globen mit einem neuartigen 3D-gedruckten Halter

Article DOI: 10.3791/64510-v • 05:41 min • October 6th, 2022
October 6th, 2022 •
*1, *2, 2, 3, 2,3
1Department of Pharmacology, School of Medicine, Boston University, 2Department of Biochemistry, School of Medicine, Boston University, 3Department of Ophthalmology, School of Medicine, Boston University
* These authors contributed equally

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Die vorliegende Arbeit beschreibt ein neuartiges experimentelles Protokoll, das einen 3D-gedruckten Halter verwendet, um hochauflösende Lebendzellbildgebung von enukleierten Globen zu ermöglichen. Durch dieses Protokoll kann die zelluläre Kalziumsignalaktivität in verwundetem Hornhautepithel von ex vivo Globen in Echtzeit beobachtet werden.

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Biochemie Ausgabe 188
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