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大肠杆菌抗生素持久性的群体和单细胞分析
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
DOI:

12:29 min

March 24, 2023

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Chapters

  • 00:05Introduction
  • 00:31Cell Culture and Growth Curve
  • 02:21Determination of Minimal Inhibitory Concentration of Antibiotics
  • 04:03Time‐Kill Assay
  • 06:24Microfluidic Time‐Lapse Microscopy Imaging
  • 09:57Results: Population and Single‐Cell Analysis of Antibiotic Persistence
  • 11:47Conclusion

Summary

자동 번역

抗生素持久性描述了敏感等基因人群中的小亚群暂时耐受高剂量杀菌抗生素的能力。本协议结合了在将 大肠杆菌 暴露于致死剂量的氧氟沙星后在分子和细胞水平上表征抗生素持久性表型的方法。

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