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Journal
/
Biology
/
ヒドロゲル中でのオルガノイドおよびスフェロイド全体の培養、凍結、加工、イメージング
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JoVE Journal
Biology
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
Please note that all translations are automatically generated.
Click here for the English version.
ヒドロゲル中でのオルガノイドおよびスフェロイド全体の培養、凍結、加工、イメージング
DOI:
10.3791/64563-v
•
08:07 min
•
December 23, 2022
•
Olgu Enis Tok
2
,
Gamze Demirel
3
,
Yusuf Saatci
,
Zeynep Akbulut
5
,
Ozgecan Kayalar
,
Ranan Gulhan Aktas
3,4
1
Research Institute for Health Sciences and Technologies (SABITA)
,
Istanbul Medipol University
,
2
Cellorama
,
3
Cancer and Stem Cell Research Center
,
Maltepe University
,
4
Department of Histology and Embryology, School of Medicine
,
Maltepe University
,
5
Department of Medical Biology, School of Medicine
,
Maltepe University
,
6
School of Medicine
,
Koc University
Chapters
00:04
Introduction
01:14
Culturing of the Organoids/Spheroids
02:27
Immunofluorescence Labeling of Whole-Mount Organoids/Spheroids in a Hydrogel
04:43
Freezing and Thawing of Whole-Mount Organoids/Spheroids in a Hydrogel
05:53
Results: Investigating the Whole-Mount Organoids/Spheroids in Hydrogel by Imaging
07:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究では、スフェロイドおよびオルガノイド全体を多目的デバイス内のヒドロゲルにそのまま残したまま、さまざまな顕微鏡で培養、凍結、解凍、処理、染色、標識、および検査するための方法論について説明します。
Tags
Organoids
Spheroids
Hydrogel
3D Culture
Freezing
Thawing
Processing
Imaging
Microscopy
Disease Modeling
Biomarkers
HEPG2 Cells
Immunofluorescence Labeling
Fixation
Washing
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