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ヒドロゲル中でのオルガノイドおよびスフェロイド全体の培養、凍結、加工、イメージング
JoVE Journal
Biology
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JoVE Journal Biology
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
DOI:

08:07 min

December 23, 2022

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Chapters

  • 00:04Introduction
  • 01:14Culturing of the Organoids/Spheroids
  • 02:27Immunofluorescence Labeling of Whole-Mount Organoids/Spheroids in a Hydrogel
  • 04:43Freezing and Thawing of Whole-Mount Organoids/Spheroids in a Hydrogel
  • 05:53Results: Investigating the Whole-Mount Organoids/Spheroids in Hydrogel by Imaging
  • 07:25Conclusion

Summary

Automatic Translation

本研究では、スフェロイドおよびオルガノイド全体を多目的デバイス内のヒドロゲルにそのまま残したまま、さまざまな顕微鏡で培養、凍結、解凍、処理、染色、標識、および検査するための方法論について説明します。

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