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Virus Propagation and Cell-Based Colorimetric Quantification
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Virus Propagation and Cell-Based Colorimetric Quantification

Virus Propagation and Cell-Based Colorimetric Quantification

DOI:
10.3791/64578-v

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07:26 min

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April 07, 2023

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, , , ,
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE),Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine,Universiti Malaya

Chapters

  • 00:00Introduction
  • 00:43Virus Propagation
  • 02:15Virus Quantification
  • 04:31Staining
  • 05:29Results: Foci Forming Assay for Zika Virus P6-740 in 24 and 96-Well Plates
  • 06:55Conclusion

Summary

자동 번역

자동 번역

The present protocol describes the propagation of Zika virus (ZIKV) in Vero African green monkey kidney cells and the quantification of ZIKV using cell-based colorimetric immunodetection methods in 24-well and 96-well (high throughput) formats.

Tags

Keywords: Virus PropagationCell-based Colorimetric QuantificationZika VirusAntibody RecognitionVirus SerotypesHigh-throughput ApplicationsAutomated Imaging SystemVero CellsCell CultureVirus InoculumVirus QuantificationSerial Dilution

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