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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
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JoVE 신문 생물학
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
DOI:

07:48 min

October 11, 2022

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Chapters

  • 00:05Introduction
  • 00:42Time-Lapse Fluorescence Microscopy
  • 05:32Analysis of Chromatin Segregation
  • 06:18Results: Ctf19 Anchor Away Time and Chromatin Segregation
  • 07:10Conclusion

Summary

자동 번역

Time-lapse microscopy is a valuable tool for studying meiosis in budding yeast. This protocol describes a method that combines cell-cycle synchronization, time-lapse microscopy, and conditional depletion of a target protein to demonstrate how to study the function of a specific protein during meiotic chromosome segregation.

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