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从人类扩增的潜在干细胞中产生自然杀伤细胞

JoVE 신문
발생학

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Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

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从人类扩增的潜在干细胞中产生自然杀伤细胞

DOI:

10.3791/64608-v

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06:53 min

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January 13, 2023

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Poon Yu Ching, Clement Wang, Su Hang, Pentao Liu³, Ryohichi Sugimura³

¹Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, ²School of Biomedical Sciences,The University of Hong Kong, ³Centre for Translational Stem Cell Biology

Chapters

00:04Introduction
00:33Generation of 3D Organoid Structures From hEPSCs
02:02Mesoderm and Hemato-Endothelial Patterning of the Embryoid Body
03:07NK Cell Differentiation
05:05Results: Analysis of In Vitro Differentiation (IVD) Products
06:29Conclusion

Summary

자동 번역

本协议显示了如何在3D和2D培养条件下区分具有轻度细胞毒性的CD3 - / CD45 + CD56 +细胞与人扩增潜在干细胞（hEPSC）。这允许在不破坏复杂微环境的情况下进行常规表型验证。

Tags

Keywords: Natural Killer Cells Human Expanded Potential Stem Cells Cancer Immunotherapy Embryoid Bodies Mesoderm Patterning Hematoendothelial Specification Differentiation Cell Culture Cell Suspension Cell Counting Cell Harvesting

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