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Detektion av polyfunktionella T-celler hos barn vaccinerade med japanskt encefalitvaccin via flödescytometritekniken
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children,2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine,Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing),Becton Dickinson Medical Devices (Shanghai) Co Ltd
Chapters
- 00:05Introduction
- 00:39Stimulation of PBMCs by Inactivated JEV Particles to Induce Cytokine Expression and Ex Vivo Intracellular Staining
- 04:28Flow Cytometry Set-Up, Gating Strategy, and Data Analysis
- 06:56Results: Flow Cytometry Analysis of Polyfunctional T Cell Profiles in PBMCs Within Japanese Encephalitis Virus Vaccinated Children
- 09:12Conclusion
Detta protokoll kombinerar ex vivo-stimulering och flödescytometri för att analysera polyfunktionella T-cellprofiler (TPF) i mononukleära celler i perifert blod (PBMC) inom japanskt encefalitvirus (JEV) -vaccinerade barn. Detektionsmetoden och flödescytometrifärgschemat för JEV-specifika TPF testades för att ge en referens för liknande studier.
