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Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

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Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

DOI:

10.3791/64688-v

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05:34 min

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February 10, 2023

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Peibo Wang*¹, Yongcan Zhou*^1,3, Bei Wang, Ahmed Elaswad, Shifeng Wang³, Weiliang Guo³, Dongdong Zhang³

¹State Key Laboratory of Marine Resource Utilization in South China Sea,Hainan University, ²Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery,Guangdong Ocean University, ³Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science,Hainan University, ⁴Department of Animal Wealth Development, Faculty of Veterinary Medicine,Suez Canal University

Chapters

00:05Introduction
00:42Fish, Reagent, and Equipment Preparation
01:18Tissue Dissection and Cell Dissociation
03:50Staining and Microscopic Examination
04:16Results: Effect of Enzymes on Tissue Dissociation
04:52Conclusion

Summary

자동 번역

Here, we demonstrate the preparation of a high-quality single-cell suspension of tilapia intestine for single-cell sequencing.

Tags

Keywords: Single-cell Suspension Nile Tilapia Intestine Single-cell Sequencing Enzymatic Cell Dissociation Collagenase Dispase PBS FBS Intestinal Contents Mucus BSA-DPBS RNA Inhibitor Cell Strainer

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