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Chapters
- 00:04Introduction
- 01:03Preparation of ConA‐Sepharose Beads and ConA‐Sepharose: Amylopectin Beads
- 02:19Starch Excess4 (SEX4) Protein Binding with ConA‐Sepharose: Amylopectin Beads
- 03:41Running SDS‐PAGE Gels
- 04:27Western Blotting for Chemiluminescence Detection and Data Analysis
- 06:28Results: Binding Affinity of SEX4 to ConA‐Sepharose‐Amylopectin Beads
- 08:18Conclusion
This method describes a lectin-based in vitro sedimentation assay to quantify the binding affinity of glucan phosphatase and amylopectin. This co-sedimentation assay is reliable for measuring glucan phosphatase substrate binding and can be applied to various solubilized glucan substrates.
