This content is Free Access.
1Nutrition and Microbiome Laboratory, Institut du cancer de Montréal,Centre de recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Digestive Surgery Service, Centre hospitalier de l’Université de Montréal (CHUM), Faculty of Medicine,Université de Montréal, 3Department of Medicine, Faculty of Medicine,Université de Montréal
Chapters
- 00:05Introduction
- 00:39Administration of FITC-Dextran
- 01:09In Vivo Fluorescence Measurement
- 02:44Fluorescence Measurement in Fecal Samples and Plasma
- 03:50Results: Dietary Supplementation with Inulin and the Translocation of FITC-Dextran
- 04:45Conclusion
I denne studien administreres fluorescein-isotiocyanatmerket (FITC) dextran til mus via oral gavage for å evaluere intestinal permeabilitet både in vivo og i plasma- og fekalprøver. Siden tarmbarrierefunksjonen påvirkes i mange sykdomsprosesser, kan denne direkte og kvantitative analysen brukes i ulike forskningsområder.
