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Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
 

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

Article DOI: 10.3791/64761-v • 07:59 min • October 28th, 2022
October 28th, 2022 •
1, 1, 1, 1, 1
1Ocular and Stem Cell Translational Research Section, National Eye Institute, National Institutes of Health (NIH)

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요약
Automatic Translation

We describe a method to efficiently separate retinal pigment epithelium (RPE) from the retina in human eyes and generate whole RPE/choroid flatmounts for histological and morphometric analyses of the RPE.

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Keywords: Retinal Pigment Epithelium RPE Choroid Flatmount Histological Analysis Retinal Degeneration Dissection Technique REShAPE Software D-Mannitol DPBS PFA Macular Region Ciliary Margin
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