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Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
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Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

DOI:
10.3791/64769-v

•

13:39 min

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July 07, 2023

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1Laboratory of Membrane Biology and Biophysics,The Rockefeller University

Chapters

  • 00:04Introduction
  • 00:47Preparations
  • 01:59Inside the Biological Safety Cabinet
  • 04:16Working with Adherent Cells
  • 06:13Checking and Storing Cells
  • 07:19Working with Suspension Cells
  • 08:52Cell Incubation
  • 09:05Liquid Waste Collection
  • 09:29Clean Up
  • 10:38Organization
  • 11:13Representative Results
  • 12:42Conclusion

Summary

자동 번역

자동 번역

This protocol presents essential cell culture techniques and practices to be used in the research cell culture laboratory to avoid contamination by fungi and bacteria. Within the category of bacteria, special emphasis will be placed on preventing mycoplasma contamination.  

Tags

Keywords: Cell CultureContaminationFungiBacteriaMycoplasmaLaboratoryTechniquesPracticesLab CoatGlovesBiological Safety Cabinet70% EthanolWater BathMedia BottleAir FlowFiltration

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