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1Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine
Chapters
- 00:04Introduction
- 01:03Collection, Preparation, and Extraction of Plasma Sample
- 03:28ELISA Plate Preparation (Blocking)
- 04:16Enzyme-Linked Immunosorbent Assay (ELISA)
- 06:06Results: Effect of Blocking on CGRP Quantification
- 06:29Conclusion
Gepubliceerde gegevens met betrekking tot calcitonine gen-gerelateerde peptide (CGRP) concentraties in menselijk plasma zijn inconsistent. Deze inconsistenties kunnen te wijten zijn aan het ontbreken van een gestandaardiseerde, gevalideerde methodologie om dit neuropeptide te kwantificeren. Hier beschrijven we een gevalideerd enzyme-linked immunosorbent assay (ELISA) protocol om CGRP in menselijk plasma te zuiveren en te kwantificeren.
