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离体聚乙烯醇基培养物中小鼠造血干细胞的扩增和遗传操作

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Ex Vivo Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures

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离体聚乙烯醇基培养物中小鼠造血干细胞的扩增和遗传操作

DOI:

10.3791/64791-v

•

07:55 min

•

February 10, 2023

•

Hwei Minn Khoo*¹, Grace A. Meaker*¹, Adam C. Wilkinson

¹MRC Weatherall Institute of Molecular Medicine,University of Oxford

Chapters

00:05Introduction
01:05Bone Marrow Extraction and c-Kit^&#43 Enrichment of Hematopoietic Stem Cell &#40HSC&#41
03:03Initiating Cell Cultures with c-Kit-Enriched Hematopoietic Stem and Progenitor Cells &#40HSPCs&#41
03:45Performing Media Changes
04:19Electroporating Cultured HSPCs
05:24Transducing Cultured HSPCs with Lentiviral Vector
06:17Results: Fluorescence-Activated Cell Sorting &#40FACS&#41 of the c-Kit-Enriched HSCs
07:22Conclusion

Summary

자동 번역

这里介绍的是使用离体聚乙烯醇基扩增启动、维持和分析小鼠造血干细胞培养物的方案，以及通过慢病毒转导和电穿孔进行遗传操作的方法。

Tags

Keywords: Hematopoietic Stem Cells Ex Vivo Expansion Genetic Manipulation Polyvinyl Alcohol Mouse Bone Marrow C-Kit Magnetic Column Separation Cell Culture

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