Visualizing Early Infection Sites of Rice Blast Disease (<em>Magnaporthe oryzae</em>) on Barley (<em>Hordeum vulgare)</em> Using a Basic Microscope and a Smartphone

Jessica G. Cooper; Nicole M. Donofrio; Jeffrey L. Caplan; Timothy R. Chaya

doi:10.3791/64794

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Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone

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Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone

Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone

DOI:

10.3791/64794-v

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07:36 min

•

March 17, 2023

•

Jessica G. Cooper, Nicole M. Donofrio, Jeffrey L. Caplan, Timothy R. Chaya

¹Department of Plant and Soil Sciences,University of Delaware

Chapters

00:04Introduction
00:40Infecting Barley (Hordeum vulgare) with Rice Blast Fungus (Magnaporthe oryzae)
03:11Staining the Infected Leaf Sheaths
04:12Mounting and Imaging of the Stained Leaf Sheaths
05:51Results: Infected and Stained Sheaths Imaging
06:57Conclusion

Summary

자동 번역

This is a straightforward protocol of a barley leaf sheath assay using minimal reagents and common laboratory equipment (including a basic smartphone). The purpose is to visualize the early infection process of blast disease in labs without access to advanced microscopy equipment.

Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone

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