Journal
/
생화학
/
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

DOI:
10.3791/64820-v

•

10:34 min

•

December 09, 2022

•

,
1Department of Microbiology,Harvard Medical School

Chapters

  • 00:04Introduction
  • 00:36Gene‐Specific In Vitro DMS Modification
  • 02:49Gene‐Specific RT‐PCR of Modified RNA
  • 04:19Whole‐Genome DMS‐MaP Using Virus‐Infected Cells
  • 07:17Analysis of the Sequencing Data
  • 08:12Results: Probing RNA Structure with Dimethyl Sulfate Mutational Profiling and Sequencing
  • 10:00Conclusion

Summary

자동 번역

자동 번역

The protocol provides instruction for modifying RNA with dimethyl sulfate for mutational profiling experiments. It includes in vitro and in vivo probing with two alternative library preparation methods.

Tags

Keywords: RNA StructureDMS-MaPSequencingIn VitroIn CellsRNA Structure EnsembleBiological ResearchRefolding BufferDimethyl SulfateBeta-mercaptoethanolSpectrophotometerTGIRTComplementary DNAPCR

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/64820?article_type=v

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
check_url/kr/64820?article_type=v

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
check_url/kr/64820?article_type=v

Polysome Profiling in Leishmania, Human Cells and Mouse Testis
check_url/kr/64820?article_type=v

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
check_url/kr/64820?article_type=v

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
check_url/kr/64820?article_type=v

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
check_url/kr/64820?article_type=v

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
check_url/kr/64820?article_type=v

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
check_url/kr/64820?article_type=v

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
check_url/kr/64820?article_type=v

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Read Article