Journal
/
/
Sondeo de la estructura del ARN con perfil mutacional de sulfato de dimetilo con secuenciación in vitro y en células
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
DOI:

10:34 min

December 09, 2022

,

Chapters

  • 00:04Introduction
  • 00:36Gene‐Specific In Vitro DMS Modification
  • 02:49Gene‐Specific RT‐PCR of Modified RNA
  • 04:19Whole‐Genome DMS‐MaP Using Virus‐Infected Cells
  • 07:17Analysis of the Sequencing Data
  • 08:12Results: Probing RNA Structure with Dimethyl Sulfate Mutational Profiling and Sequencing
  • 10:00Conclusion

Summary

자동 번역

El protocolo proporciona instrucciones para modificar el ARN con sulfato de dimetilo para experimentos de perfiles mutacionales. Incluye sondeo in vitro e in vivo con dos métodos alternativos de preparación de bibliotecas.

Related Videos

Read Article