JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Introduction
- 00:35Differentiation of hPSCs into Pancreatic Progenitor Cells
- 02:48Aggregation into Pancreatic Progenitor Clusters Using 400 μm Diameter Microwell Plates
- 04:20Differentiation of Kit-Derived Pancreatic Progenitor Clusters into hPSC-Islets
- 06:07Results: Generation of hPSC-Islets Capable of Secreting Insulin upon Glucose Challenge
- 08:07Conclusion
Tags
Keywords:
Human Pluripotent Stem CellsInsulin-producing Islet ClustersStatic Suspension CulturePancreatic ProgenitorsIslet-like ClustersHESC-qualified MatrigelCell DissociationStem Cell CultureStage 1A MediumStage 1B MediumStage 2A MediumStage 2B MediumStage 3 MediumStage 4 MediumAnti-adherence Rinsing SolutionMicrowell Plate
