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Chapters
- 00:05Introduction
- 00:53Molecular Cloning
- 04:34In Vivo Injection of Adeno-Associated Virus (AAV) into the Inguinal White Adipose Tissue (iWAT)
- 06:14In Vitro Differentiation of Stromal Vascular Cells (SVFs) into Beige Adipocytes
- 07:23In Vitro AAV Infection of SVFs
- 08:11Results: SPH-Induced Expression of Endogenous Prdm16 for Enhanced Oxygen Consumption
- 09:23Conclusion
This protocol presents the use of CRISPR SunTag-p65-HSF1 (SPH) in adipocytes (AdipoSPH) as an alternative strategy to adeno-associated virus (AAV) for investigating beige fat biology. In vivo injection of AAV-carrying sgRNA targeting the endogenous Prdm16 gene is sufficient to induce beige fat development and enhance the thermogenic gene program.
