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胚胎和肝脏斑马鱼细胞系球状体的3D培养方法
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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
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胚胎和肝脏斑马鱼细胞系球状体的3D培养方法

DOI:
10.3791/64859-v

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08:00 min

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January 20, 2023

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, , , , , , , , ,
1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

Chapters

  • 00:05Introduction
  • 00:55Cell Culture Medium and Monolayer Cultures
  • 02:20Cell Counting with Trypan Blue Dye Exclusion Test
  • 03:07Cell Plating in ULA-Plates
  • 04:48Results: Characterization of Spheroids Developed with 3D Spheroid Method
  • 07:12Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出了一种有效，简单和快速的3D培养方案，用于形成两种斑马鱼（Danio rerio）细胞系的球状体：ZEM2S（胚胎）和ZFL（正常肝细胞）。

Tags

Keywords: 3D Cell CultureZebrafish Cell LinesSpheroidsIn Vitro ModelToxicity TestingCell ViabilityTrypan BlueCell Counting96-well ULA Plate

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