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1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education,Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes,Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center,Anhui University of Science and Technology
Chapters
- 00:04Introduction
- 01:07Preparing the Crystalline Silica Dust (CSD) Suspension
- 01:52Administering the Nasal Drips to the Mouse
- 02:24Performing Hematoxylin and Eosin (HE) Staining
- 03:23Performing Masson Staining and Sirius Red Staining
- 04:27Performing Immunohistochemistry
- 06:10Performing Western Blotting Analysis
- 08:02Results: Investigating the Development of Silicosis in Mice After Repeated CSD Inhalation
- 09:41Conclusion
Ce protocole décrit une méthode permettant d’établir un modèle murin de silicose par exposition répétée à des suspensions de silice par écoulement nasal. Ce modèle peut imiter de manière efficace, pratique et flexible le processus pathologique de la silicose humaine avec une répétabilité et une économie élevées.
