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The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation
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The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation
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The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation

DOI:
10.3791/64867-v

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05:38 min

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June 23, 2023

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, , , ,
1Gynecology Research Unit, Institut de Recherche Expérimentale et Clinique,Université Catholique de Louvain, 2Gynecology Department,Cliniques Universitaires Saint-Luc

Chapters

  • 00:00Introduction
  • 00:57Preparing the Eggs for Incubation and Opening the Eggshell on Day 3 of Chick Embryo Development (ED)
  • 02:38Grafting Frozen-Thawed Human Ovarian Tissue to the CAM and Harvesting the Grafts
  • 03:33Results: Development of a CAM Xenografting Model for Human Ovarian Tissue
  • 04:37Conclusion

Summary

자동 번역

자동 번역

Here, we describe a protocol for developing a chick chorioallantoic membrane (CAM) xenografting model for human ovarian tissue and demonstrate the effectiveness of the technique, the graft revascularization time frame, and the tissue viability across a 6 day grafting period.

Tags

Keywords: Chick Chorioallantoic Membrane (CAM) ModelNon-embryonated EggsEgg CandlerEgg ManipulationCAM VascularizationFollicle LossEther-extracted Lens Paper

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