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Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures
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Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

DOI:
10.3791/64905-v

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11:58 min

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January 06, 2023

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, ,
1Department of Cell and Molecular Biology,Karolinska Institutet

Chapters

  • 00:05Introduction
  • 01:07Isolation of Esophageal Progenitor Cells and Fibroblasts
  • 05:02Establishment and Culture of Esophageal Organoids
  • 06:31Organoid Processing for Whole Mount Staining
  • 09:22Results: Isolation of Progenitor Cells and Fibroblast Subpopulations, Organoid Co-Cultures, and Whole-Mount Staining of Fibroblast-Organoid Interactions
  • 11:24Conclusion

Summary

자동 번역

자동 번역

Organoid-fibroblast co-cultures provide a model to study the in vivo stem cell niche. Here, a protocol for esophageal organoid-fibroblast co-cultures is described. Additionally, whole mount imaging is used to visualize the fibroblast-organoid interaction.

Tags

Keywords: Esophageal FibroblastsOrganoid Co-culturesProgenitor Cell NicheEsophageal OrganoidsCancer-associated FibroblastsCell DissociationEpithelial-stromal InteractionsOrganoid MorphologyFunctional Characterization

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