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Funktionel karakterisering og visualisering af esophageal fibroblaster ved hjælp af organoide co-kulturer
JoVE 신문
생물학
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JoVE 신문 생물학
Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures
DOI:

11:58 min

January 06, 2023

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Chapters

  • 00:05Introduction
  • 01:07Isolation of Esophageal Progenitor Cells and Fibroblasts
  • 05:02Establishment and Culture of Esophageal Organoids
  • 06:31Organoid Processing for Whole Mount Staining
  • 09:22Results: Isolation of Progenitor Cells and Fibroblast Subpopulations, Organoid Co-Cultures, and Whole-Mount Staining of Fibroblast-Organoid Interactions
  • 11:24Conclusion

Summary

자동 번역

Organoid-fibroblast co-kulturer giver en model til at studere in vivo stamcelleniche. Her beskrives en protokol for esophageal organoid-fibroblast co-kulturer. Derudover bruges helmonteret billeddannelse til at visualisere fibroblast-organoid-interaktionen.

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