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1Department of Chemical and Biomolecular Engineering, Samueli School of Engineering,University of California, Irvine, 2Department of Biomedical Engineering, Samueli School of Engineering,University of California, Irvine, 3Department of Chemistry, School of Physical Sciences,University of California, Irvine, 4Sue & Bill Gross Stem Cell Research Center,University of California, Irvine
Chapters
- 00:05Introduction
- 00:50Light-Induced Dielectrophoresis (LiDEP) Chip Device Fabrication
- 03:05Dielectrophoresis (DEP) Buffer Preparation
- 03:55Cell Preparation
- 04:40Light-Induced Dielectrophoresis (LiDEP) Characterization
- 07:41Results: Dielectrophoresis Responses in hMSCs
- 09:28Conclusion
Her presenterer vi lysindusert dielektroforese som en etikettfri tilnærming for å karakterisere heterogene cellelinjer, spesielt humane mesenkymale stamceller (hMSCs). Dette papiret beskriver en protokoll for bruk og optimalisering av en mikrofluidisk enhet med et fotoledende lag for å karakterisere den elektriske oppførselen til hMSC uten å endre deres opprinnelige tilstand.
