This content is Free Access.
1Department of Chemical and Biomolecular Engineering, Samueli School of Engineering,University of California, Irvine, 2Department of Biomedical Engineering, Samueli School of Engineering,University of California, Irvine, 3Department of Chemistry, School of Physical Sciences,University of California, Irvine, 4Sue & Bill Gross Stem Cell Research Center,University of California, Irvine
Chapters
- 00:05Introduction
- 00:50Light-Induced Dielectrophoresis (LiDEP) Chip Device Fabrication
- 03:05Dielectrophoresis (DEP) Buffer Preparation
- 03:55Cell Preparation
- 04:40Light-Induced Dielectrophoresis (LiDEP) Characterization
- 07:41Results: Dielectrophoresis Responses in hMSCs
- 09:28Conclusion
Aquí, presentamos la dielectroforesis inducida por la luz como un enfoque sin marcado para caracterizar líneas celulares heterogéneas, específicamente células madre mesenquimales humanas (hMSC). Este artículo describe un protocolo para usar y optimizar un dispositivo microfluídico con una capa fotoconductora para caracterizar el comportamiento eléctrico de las hMSC sin alterar su estado nativo.
