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评估和量化年龄相关性黄斑变性小鼠模型中视网膜色素上皮病理的协议

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A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

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评估和量化年龄相关性黄斑变性小鼠模型中视网膜色素上皮病理的协议

DOI:

10.3791/64927-v

•

09:24 min

•

March 10, 2023

•

Michael Landowski², Samuel Grindel, Ying Hao, Sakae Ikeda², Catherine Bowes Rickman⁴, Akihiro Ikeda²

¹Department of Medical Genetics,University of Wisconsin-Madison, ²McPherson Eye Research Institute,University of Wisconsin-Madison, ³Department of Ophthalmology,Duke University, ⁴Department of Cell Biology,Duke University

Chapters

00:04Introduction
01:04Evaluation of Mouse Retinal Pigmented Epithelium (RPE) by Light Microscopy
05:29Evaluation of Mouse RPE Through Confocal Microscopy
06:32Results: Investigating RPE Pathologies in Mice
08:35Conclusion

Summary

자동 번역

小鼠模型可以成为研究视网膜色素上皮（RPE）生物学的有用工具。已经确定小鼠可以发展出一系列RPE病理。在这里，我们描述了一种表型方案，以使用光，透射电子和共聚焦显微镜阐明和量化小鼠的RPE病理。

Tags

Keywords: Retinal Pigmented Epithelium Age-related Macular Degeneration Mouse Model Light Transmission Electron Microscopy Confocal Microscopy Cardiac Perfusion Eye Nucleation

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