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Echtzeitmessung des mitochondrialen bioenergetischen Profils von Neutrophilen
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
DOI:

09:39 min

June 02, 2023

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Chapters

  • 00:05Introduction
  • 01:05Preparation of the 96-Well Plate for the Metabolic Extracellular Flux Assay
  • 01:52Preparation and Seeding of Cells
  • 03:52Preparing Compounds in the Mitochondrial Stress Test Kit
  • 04:25Running the Mitochondrial Stress Assay
  • 07:29Results: Metabolic Flux Assay to Analyze Oxygen Consumption Rates (OCR) in Neutrophils
  • 09:02Conclusion

Summary

자동 번역

Wir beschreiben schrittweise Protokolle zur Messung der mitochondrialen Atmung von Maus und humanen Neutrophilen und HL60-Zellen mit dem metabolischen extrazellulären Flussanalysator.

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