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Neuroscience

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Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
 

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

Article DOI: 10.3791/64989-v 09:39 min March 17th, 2023
March 17th, 2023

챕터

요약

Here, we present a protocol to perform a whole-cell patch-clamp on brain slices containing kisspeptin neurons, the primary modulator of gonadotrophin-releasing hormone (GnRH) cells. By adding knowledge about kisspeptin neuron activity, this electrophysiological tool has served as the basis for significant advancements in the neuroendocrinology field over the last 20 years.

Tags

Keywords: Whole-cell Patch-clamp Recordings Electrophysiological Properties Cell Membranes Ionic Currents Cellular Response Brain Dissection Hypothalamus Optic Nerve Vibratome ACSF Solution Microscope Amplifier Digitizer Micromanipulator Perfusion Pump
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