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Chapters
- 00:05Introduction
- 01:34Fabricating the Master Wafer and Preparing the Microfluidic Device
- 03:46Making the PDMS-Coated Glass Slide and Bonding the Microfluidic Device
- 05:24Surface Functionalization of the Microfluidic Device
- 06:48Liposome Production
- 07:33Results: Production of Monodispersed Liposomes and Subsequent pH-Triggered Liquid-Liquid Phase Separation of pLL/ATP Inside Liposomes
- 09:02Conclusion
The present protocol describes octanol-assisted liposome assembly (OLA), a microfluidic technique to generate biocompatible liposomes. OLA produces monodispersed, micron-sized liposomes with efficient encapsulation, allowing immediate on-chip experimentation. This protocol is anticipated to be particularly suitable for synthetic biology and synthetic cell research.
Tags
Keywords:
On-chip Liposome AssemblyOLAMicrofluidic PlatformSynthetic BiologyArtificial CellsSelf-organizationCell-mimicking AssembliesMonodispersed LiposomesHigh-throughputEncapsulation EfficiencyBiological ReactionsVesicle DynamicsBiomolecular CondensatesMembrane InteractionsDrug ScreeningAntimicrobial TestingSurface FunctionalizationPhotolithography
