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通过冷冻STEM断层扫描原位可视化细胞器

JoVE 신문
생물학

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JoVE 신문 생물학

Visualization of Organelles In Situ by Cryo-STEM Tomography

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通过冷冻STEM断层扫描原位可视化细胞器

DOI:

10.3791/65052-v

•

08:37 min

•

June 23, 2023

•

Peter Kirchweger, Debakshi Mullick, Sharon Grayer Wolf, Michael Elbaum

¹Department of Chemical and Biological Physics,Weizmann Institute of Sciences, ²Department of Chemical Research Support,Weizmann Institute of Sciences

Chapters

00:05Introduction
00:48STEM Setup
04:37Placing the Specimen and Recording an Image
06:58Recording an Image
07:23Results: Visualization of the Organelles
08:03Conclusion

Summary

자동 번역

冷冻 STEM 断层扫描提供了一种可视化完整细胞细胞器的方法，无需包埋、切片或其他侵入性制剂。获得的3D分辨率目前在几纳米的范围内，视场为几微米，可访问厚度约为1微米。

Tags

Cryo-STEM Tomography TEM Tomography FIB SEM Soft X-ray Tomography STEM Optics Column Alignment Eucentric Focus STEM Mode Condenser Aperture Beam Shift Rotation Center Pivot Points Condenser Stigmators

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