Journal
/
/
A GMP-Compliant Procedure for the Generation of Gene-Modified T cells
JoVE 신문
생체공학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생체공학
A GMP-Compliant Procedure for the Generation of Gene-Modified T cells
DOI:

06:47 min

October 06, 2023

, , , , , , ,

Chapters

  • 00:00Introduction
  • 00:38Day 0: Peripheral Blood Mononuclear Cells (PBMC) Isolation and T Cell Activation
  • 01:42Day 3: Lentiviral T Cell Transduction
  • 03:04Day 4: Virus Removal and T Cell Expansion
  • 03:44Day 14: Cryopreservation
  • 04:39Transduction Efficiency-Flow Cytometry Staining (Day 14)
  • 05:35Results: Assessment of the Transduced Human T Cells
  • 06:29Conclusion

Summary

자동 번역

This protocol outlines the process of transducing primary human T cells with a gene of interest, ensuring compatibility with Good Manufacturing Practice (GMP) standards.

Related Videos

Read Article