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通过PINK1 / Parkin途径介导的线粒体自噬 保护 H9c2心肌细胞免受藏红花素氧化应激
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Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
DOI:

07:40 min

May 26, 2023

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Chapters

  • 00:05Introduction
  • 00:43Cell Viability Determination
  • 02:05Preparation for ROS and Mitochondrial Membrane Potential Determination
  • 03:22TUNEL Staining Assay for Apoptosis Rate Determination
  • 04:10Transfection of mCherry GFP-LC3B Adenovirus to Monitor Autophagic Flow
  • 04:53Immunofluorescence Staining for Detection of Parkin’s Mitochondrial Translocation
  • 05:42Results: Effects of Crocetin on Oxidative Stress-Induced Damage in H9c2 Cardiomyocytes
  • 07:10Conclusion

Summary

자동 번역

本研究在体外实验的基础上揭示了藏红花素通过影响线粒 自噬修复心肌细胞氧化应激损伤的机制,其中PINK1/Parkin信号通路起重要作用。

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