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芳香族氨基酸调控细胞代谢的生物正交化学成像
JoVE 신문
생체공학
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JoVE 신문 생체공학
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
DOI:

10:42 min

May 12, 2023

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Chapters

  • 00:05Introduction
  • 00:54Media Preparation
  • 02:10Cell Sample Preparation
  • 05:22Spontaneous Raman Spectroscopy Measurement
  • 06:15Imaging Experiments with Two-Photon Excitation Fluorescence and Stimulated Raman Scattering
  • 08:25Results: Visualizing Metabolic Dynamics and 3D Lipid Droplet Distribution in HeLa Cells
  • 09:48Conclusion

Summary

자동 번역

我们提出了一种协议,使用氧化氘(重水D2O)探测的受激拉曼散射(DO-SRS)显微镜直接可视化由氨基酸调节的细胞中的代谢活动,该显微镜与双光子激发荧光显微镜(2PEF)集成。

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