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生成用于药物评估研究的 3D 肿瘤微球

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Generation of 3D Tumor Spheroids for Drug Evaluation Studies

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生成用于药物评估研究的 3D 肿瘤微球

DOI:

10.3791/65125-v

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10:33 min

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February 24, 2023

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Wei Hou*^1,2, Zilin Zhang*^3,4, Jing Zhang⁴, Shihui Xu⁴, Menglan Li, Xiaoran Li⁴, Zaozao Chen³, Cailian Wang²

¹Department of Oncology, School of Medicine,Southeast University, ²State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering,Southeast University, ³Institute of Medical Devices (Suzhou),Southeast University, ⁴Jiangsu Avatarget Biotechnology Co., Ltd.

Chapters

00:04Introduction
00:58Spheroid Construction
05:38Drug Treatment and Spheroid Viability
07:09Spheroid Observation and Deep Learning Analysis Through Images in the Drug Test
08:23Results: Brightfield Images of NCI‐H23 Cell Spheroids Treated with AMG510, Tumor Spheroid Viability, Tumor Size and Invasiveness
10:09Conclusion

Summary

자동 번역

本文演示了一种构建三维肿瘤球体的标准化方法。还描述了使用自动成像系统进行球体观察和基于图像的深度学习分析的策略。

Tags

3D Tumor Spheroids Drug Evaluation High-throughput Imaging High-content Analysis AMG510 NCI-H23 Cells Anti-adhesion Reagent Trypsin-EDTA Cell Suspension Cell Seeding Density Centrifugation

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