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枯草芽孢杆菌孢子计数和标记分析在流式细胞术中的改进
JoVE 신문
생화학
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JoVE 신문 생화학
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
DOI:

06:39 min

June 30, 2023

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Chapters

  • 00:00Introduction
  • 00:44Flow Cytometry Setting and Preparation of Autoclaved Spores for Flow Cytometry
  • 01:47Analysis Using Flow Cytometry
  • 03:27Estimation of the Protein Coupling Index on a Spore Surface Using Flow Cytometry
  • 04:52Results: Fluorescence Coupling Percentage Analysis of Bacillus subtilis Spores
  • 06:08Conclusion

Summary

자동 번역

该协议侧重于使用流式细胞术和计数珠来定量用溴化乙锭标记的细菌孢子。该方法还可用于分析完整孢子表面蛋白质的共价偶联。

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