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Chapters
- 00:04Introduction
- 00:54Polydimethylsiloxane (PDMS) Mold Generation
- 02:24PLGA Film Fabrication and Particle Generation
- 04:09Particle Filling, Sealing, and Harvesting
- 05:55Results: Effect of Particle Filling and Sealing on Antigen Encapsulation
- 07:12Conclusion
This method describes the encapsulation of the rabies antigen into biodegradable polymeric microparticles with structural and material properties that enable pulsatile release after a predetermined delay. Enzyme-linked immunosorbent assay (ELISA) assessment of the antigen retrieved from the particle core confirms the presence of intact trimeric rabies virus glycoprotein through particle fabrication.
