Journal
/
면역학 및 감염병학
/
Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time

Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time

DOI:
10.3791/65149-v

•

05:49 min

•

March 24, 2023

•

, ,
1Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy,KU Leuven

Chapters

  • 00:04Introduction
  • 00:40Cell‐Based Electrical Impedance (CEI) Plate Preparation, Cell Seeding, and CEI Assay
  • 02:12Antiviral Compound Preparation and Virus Infection
  • 03:28Data Analysis
  • 03:57Results: Effect of Antiviral Compound and Zika Virus Replication on CEI Profiles
  • 05:22Conclusion

Summary

자동 번역

자동 번역

Here, we demonstrate the use of cell-based electrical impedance (CEI) as a very easy and straightforward method to study Zika virus infection and replication in human cells in real time. Furthermore, the CEI assay is useful for the evaluation of antiviral compounds.

Tags

Cell-based Electrical ImpedanceReal-time Viral InfectionAntiviral CompoundsA549 CellsCEI AssayECIS DeviceVirus InhibitorsCell AdherenceCell Monolayer

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Zika Virus Specific Diagnostic Epitope Discovery

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

Read Article